Publications

Azadmanesh, J., Trickel, S. R., and Borgstahl, G. E. O. “Substrate-analog binding sites and electrostatic surfaces of human manganese superoxide dismutase” J. Struct. Biol. 199, 68-75 (2017).

Azadmanesh, J., Trickel, S. R., Weiss, K.L., Coates, L. and Borgstahl, G. E. O. “Neutron diffraction analysis of human manganese superoxide dismutase” Acta Crystallogr. F73, 235-240 (2017).

Mir, R.A., Lovelace, J., Schafer, N.P., Simone, P.D., Kellezi, A., Kolar, C., Spagnol, G., Sorgen, P.L., Band, H., Band, V., Borgstahl, G. E. O. "Biophysical characterization and modeling of human Ecdysoneless (ECD) protein supports a scaffolding function 3" AIMS Biophysics, 195-208 (2016).

Rohr, J., Guo, S., Huo, J., Bouska, A., Lachel, C., Li, Y., Simone, P.D., Zhang, W., Gong, Q., Wang, C., Cannon, A., Heavican, T., Mottok, A., Hung, S., Rosenwald, A., Gascoyne, R., Fu, K., Greiner, T.C., Weisenburger, D.D., Vose, J.M., Staudt, L.M., Xiao, W., Borgstahl, G.E.O. , Davis, S., Steidl, C., McKeithan, T., Iqbal, J., Chan, W.C. "Recurrent activating mutations of CD28 in peripheral T-cell lymphomas" Leukemia 1. 1-9 (2016).

Choi, K. H., Lakamp-Hawley, A. S., Kolar, C., Yan, Y., Borgstahl, G. E. O., Ouellette, M. M. “The OB-fold domain 1 of human POT1 recognizes both telomeric and non-telomeric DNA motifs” Biochimie 115, 17-27 (2015).

Borgstahl, G. E. O., Brader, K., Mosel, A., Liu, S., Kremmer, E., Goettsch, K.A., Kolar, C., Nasheuer, H.P., Oakley, G.G., "Interplay of DNA damage and cell cycle signaling at the level of human Replication Protein A" DNA Repair 21, 12-23 (2014).

Simone, P., Struble, L. R., Kellezi, A., Brown, C. A., Garbow, C. E., Khutsishvili, I., Marky, L. A., Pavlov, Y. I., and Borgstahl, G. E. O. "The human ITPA polymorphic variant P32T is destabilized by the unpacking of the hydrophobic core" J. Structural Biology 182. 197-208 (2013).

Kumar, E., Chen, Q., Kizhake, S., Kolar, C., Kang, M., Chang, C, Borgstahl, G. E. O., and Natarajan, A. "The paradox of conformational constraints in the design of Cbl(TKB)-binding peptides" Scientific Reports 3, 1639 (2013).

Simone, P., Pavlov, Y. I., and Borgstahl, G. E. O. "ITPA (inosine triphosphate pyrophatase): From surveillance of nucleotide pools to human disease and pharmacogenetics" Mutation Research 753, 131-146 (2013)

Lovelace, J.J., Simone, P.D., Petricek, V., and Borgstahl, G.E.O. "Simulation of modulated protein crystal structure and diffraction data in a supercell and in superspace" Acta Crystallographica Section D: Biological Crystallography, 2013, Volume D69, pages 1062-1072.

Kumar, E. A., Yuan, Z., Palermo, N. Y., Dong, L., Ahmad, G., Lokesh, G. L., Kolar, C., Kizahk, S., Borgstahl, G. E. O., Band, H., and Natarajan, A. "Peptide truncation leads to a twist and an unusual increase in affinity for Cbl tyrosine kinase binding domain" J. Med. Chem. 55, 3583-3597 (2012).

Mohapatra, B., Ahmad, G., Nadeau, S., Zutshi, N., Ang, W., Scheffee, S., Dong, L., Feng, D., Goetz, B., Arya, P., Baily, T. A., Palermo, N., Borgstahl, G. E. O., Natarajan, A., Raja, S., Naramura, M., Band, V., and Band, H. "Protein tyrosine kinase regulation by ubiquitination: critical roles of Cbl-family ubiquitin ligases" Molecular Cell Research 1833, 122-139 (2012).

Nadeau, S., An, W., Palermo, N., Feng, D., Ahmad, G., Dong, L., Borgstahl, G. E. O., Nararajan, A., Naramura, M., Band, V., and Band, H. "Oncogenic signaling by leukemia-associated mutant Cbl proteins" Biochemistry and Analytical Biochemistry 6, 7921 (2012).

Porta, J.C. and Borgstahl, G.E.O. "Structural Basis for Profilin-Mediated Actin Nucleotide Exchange" J. Mol. Biol. (2012) 418, 103-116.

Prakash, A. and Borgstahl, G.E.O. "The Structure and Function of Replication Protein A in DNA Replication" Invited Review for The Eukaryotic Replisome: A Guide to Protein Structure and Function, Subcellular Biochemistry 62, 103-116 (2012).

Bao, B., Pestinger, V., Hassan, Y. I., Borgstahl, G. E. O., Kolar, C., and Zempleni, J. "Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18" J. Nutr. Biochem. 22, 470-475 (2011).

Lada, A. G., Waisertreiger, I. S.-R., Grabow, C. E., Prakash, A., Borgstahl, G. E. O, Rogozin, I. B., and Pavlov, Y. I. "Replication protein A (RPA) hampers the processive action of APOBEC3G cytosine deaminase on single-stranded DNA" PLoS ONE 6, e24848 (2011).

Jason Porta, Jeffrey J. Lovelace, Antoine M. M. Schreurs, Loes M. J. Kroon-Batenburg and Gloria E. O. Borgstahl "Processing incommensurately modulated protein diffraction data with EVAL15", Acta Cryst. D67, 628-638 (2011).

Prakash, A., Kieken, F., Marky, L. A., and Borgstahl, G. E. O. "Stabilization of a G-quadruplex from unfolding by Replication Protein A using potassium and the porphyrin TMPyP4" J. Nucleic Acids 2011, PDF ID 529828, 13 pages (2011).

Prakash, A., Natarajan, A., Marky, L. A., Ouellette, M. M. and Borgstahl, G. E. O. "Identification of the DNA binding domains of human Replication Protein A that recognize G-quadruplex DNA" J. Nucleic Acids 2011, PDF ID 896947, 14 pages (2011).

Nina A. Filenko, Carol Kolar, John T. West, S. Abbie Smith, Yousef I. Hassan, Gloria E. O. Borgstahl, Janos Zempleni, Yuri L. Lyubchenko "The Role of Histone H4 Biotinylation in the Structure of Nucleosomes" Plos One 6, 1-7 (2011).

Jodi L. Kreiling, Kerry Brader, Carol Kolar, and Gloria E. O. Borgstahl "A Real-Time and Hands-On Research Course in Protein Purification and Characterization" Biochemistry and Molecular Biology Education Journal 39, 28-37 (2011).

Jeffrey J. Lovelace and Gloria E. O. Borgstahl, "The use of orthogonal projection to visualize mosaic domains from topographic data collected on protein crystals", Journal of Applied Crystallography, 2010, Volume 43, pages 907-912.

Michael G. Kemp, Aaron C. Mason, Aura Carreira, Joyce T. Reardon, Stuart J. Haring, Gloria E. O. Borgstahl, Stephen C. Kowalczykowski, Aziz Sancar and Marc S. Wold, "An Alternative Form of Replication Protein A Expressed in Normal Human Tissues Supports DNA Repair", The Journal of Biological Chemistry vol. 285, no. 7, pp. 4788-4797, February 12, 2010.

Jason Porta, Ardeschir Vahedi-Faridi and Gloria E. O. Borgstahl, "Structural Analysis of Peroxide-Soaked MnSOD Crystals Reveals Side-On Binding of Peroxide to Active-Site Manganese", J. Mol. Biol. (2010) 399, 377-384.

Jeffrey J. Lovelace, Martyn D. Winn and Gloria E. O. Borgstahl, "Simulation of modulated reflections", Journal of Applied Crystallography, 2010, Volume 43, pages 285-292 (2010).

Elena I. Stepchenkova, Elena R. Tarakhovskaya, Kathryn Spitler, Christin Frahm, Miriam R. Menezes, Peter D. Simone, Carol Kolar, Luis A. Marky, Gloria E. O. Borgstahl and Youri I. Pavlov, "Functional Study of the P32T ITPA Variant Associated with Drug Sensitivity in Humans", J. Mol. Biol. (2009) 392, 602-613.

X. Deng, A. Prakash, K. Dhar, G. S. Baia, C.l Kolar, G. G. Oakley and G. E. O. Borgstahl, "Human Replication Protein A−Rad52−Single-Stranded DNA Complex: Stoichiometry and Evidence for Strand Transfer Regulation by Phosphorylation", Biochemistry (2009).

Borgstahl, G. E. O. "Incommensurate Crystallography by Sander van Smaalen", Crystallography reviews 14 , 259-260 (2008).

Kellezi, A., Kieken, F., Grosely, R., Borgstahl, G. E. O. , and Sorgen, P. L., "Purification of the Connexin43 Carboxyl Terminus attached to the 4 th Transmembrane Domain" Protein Expression and Purification 59 , 215-222 (2008).

Lovelace, J. J., Murphy, C. R., Narayan, K., Schutt, C. E., Lindberg, U., Svensson, C., Wynn, M., and Borgstahl , G. E. O., "Protein crystals can be incommensurately modulated" J. Appl. Cryst. 41 , 600-605 (2008). Copyright © International Union of Crystallography

D. H. Juers, J. J. Lovelace, H. D. Bellamy, E. H. Snell, B. W. Mathews and G. E. O. Borgstahl, "Changes to crystals of Escherichia coli β-galactosidase during room-temperature/low-temperature cycling and their relation to cryo-annealing", Acta Cryst. (2007). D63, 1139-1153 Copyright © International Union of Crystallography.

X. Deng, J. E. Habel, V. Kabaleeswaran, E. H. Snell, M. S. Wold and G. E. O. Borgstahl, "Structure of the Full-length Human RPA14/32 Complex Gives Insights into the Mechanism of DNA Binding and Complex Formation", J. Mol. Biol. (2007) 374, 865-876.

E. Yikilmaz, J. Porta, L. E. Grove, A. Vahedi-Faridi, Y. Bronshteyn, T. C. Brunold, G. E. O. Borgstahl and A. Miller, "How Can Single Second Sphere Amino Acid Substitution Cause Reduction Midpoint Potential Changes of Hundreds of Millivolts?", J. Am. Chem. Soc., 129(32), 9927-9940, 2007.

Borgstahl, G. E. O., "How to Use of Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals" Invited Chapter in Macromolecular Crystallography Protocols, 1 , 109-129, (2006).

J. Porta, C. Kolar, S. G. Kozmin, Y. I. Pavlov and G. E. O. Borgstahl, "Structure of the orthorhombic form of human inosine triphosphate pyrophosphatase", Acta Cryst. (2006). F62, 1076-1081 Copyright © International Union of Crystallography

J. J. Lovelace, C. R. Murphy, R. Pahl, K. Brister and G. E. O. Borgstahl, "Tracking reflections through cryogenic cooling with topography", J. Appl. Cryst. (2006). 39, 425-432 Copyright © International Union of Crystallography

J. J. Lovelace and G. E. O. Borgstahl, "Ripple: a program to collect and analyze digital topographic sequences", J. Appl. Cryst. (2006). 39, 466-467 Copyright © International Union of Crystallography

J. J. Lovelace, C. R. Murphy, H. D. Bellamy, K. Brister, R. Pahl and G. E. O. Borgstahl, "Advances in digital topography for characterizing imperfections in protein crystals", J. Appl. Cryst. (2005). 38, 512-519 Copyright © International Union of Crystallography

O. A. Asojo, G. Goud, K. Dhar, A. Loukas, Bin Zhan, Vehid Deumic, Sen Liu, G. E. O Borgstahl, P. J. Hotez, "X-ray structure of Na-ASP-2, a Pathogenesis Related-1 protein from the nematode parasite Necator americanus and a vaccine antigen for human hookworm infection" J. Mol Biol., 346(3): p801-814 (2005).

F. Nachon, O. A. Asojo, G.E.O Borgstahl, P. Masson, and O. Lockridge, "Role of Water in Aging of Human Butyrylcholinesterase Inhibited by Echothiophate: The Crystal Structure Suggests Two Alternative Mechanisms of Aging" Biochemistry 44, 1154-1162 (2005).

Lovelace, J. J., Soares, A. S., Bellamy, H. D., Sweet, R. M., Snell, E. H. and Borgstahl, G. E. O. "First results of digital topography applied to macromolecular crystals" J. Appl. Cryst. (2004). 37, 481-485 Copyright © International Union of Crystallography

Lovelace, J. J., Narayan, K., Chik, J. K., Bellamy, H. D., Snell, E. H., Lindberg, U., Schutt, C. E., and Borgstahl, G. E. O. "Imaging modulated reflections from a semi-crystalline state of profilin:actin crystals" J. Appl. Cryst. (2004). 37, 327-330 Copyright © International Union of Crystallography

Vahedi-Faridi, A., Lovelace, J., Bellamy, H. D., Snell, E. H. and Borgstahl, G. E. O., "Physical and structural studies on the cryocooling of insulin crystals" Acta Cryst. (2003). D59, 2169-2182 Copyright © International Union of Crystallography

Lovelace, J. and Borgstahl, G. E. O. "BEAM-ish 2.0: a graphical user interface for the physical characterization of macromolecular crystals" J. Appl. Cryst. 36, 1101-1102 (2003).

Vahedi-Faridi, A. , Porta, J., and Borgstahl, G. E. O. "Improved three-dimensional growth of manganese superoxide dismutase crystals on the International Space Station" Acta Cryst. (2003). D59, 385-388

Snell, E. H., Bellamy, H. D., and Borgstahl, G. E. O. "Macromolecular Crystal Quality" Methods in Enzymology, Macromolecular Crystallography, Part C, editors C. Carter and B. Sweet 368, 268-288 (2003).

Jackson, D., Dhar, K., Wahl, J. K., Wold, M. S., and Borgstahl, G. E. O. "Analysis of the Human Replication Protein A:Rad52 Complex: Evidence for Crosstalk Between RPA32, RPA70, Rad52 and DNA" J. Mol. Biol. 321, 133-148 (2002).

Ranatunga, W., Jackson, D., Flowers II, R. A. and Borgstahl, G. E. O. "Human Rad52 Protein Has Extreme Thermal Stability" Biochemistry 40, 8557-8562 (2001).

Ranatunga, W., Jackson, D., Lloyd, J. A., Forget, A. L., Knight, K. L. and Borgstahl, G. E. O. "Human Rad52 Exhibits Two Modes of Self-association" J. Biol. Chem. 276, 15876-15880 (2001).

Borgstahl, G. E. O., Vahedi-Faridi, A., Lovelace, J. J., Bellamy, H. and Snell, E. "A Test of Macromolecular Crystallization in Microgravity: Large, Well-ordered Insulin Crystals" Acta Cryst. D57, 1204-1207 (2001).

Snell, E. H., Judge, R. A., Crawford, L., Pusey, M. L., Sporteillo, M., Todd, P. Bellamy, H. Lovelace, J., Cossanto, J. M. and Borgstahl, G. E., O. "Biochemical and X-ray Mosaicity Analysis of Impurity Doped Microgravity and Ground-grown Lysozyme Crystals" Crystal Growth & Design 1, 151-158 (2001).

Habel, J. E., Ohren, J. F. and Borgstahl, G. E. O. "Dynamic light scattering analysis of full-length, human RPA14/32 dimer: purification crystallization and self-association" Acta Cryst. D57, 254-259 (2001).

Bellamy, H. D., Snell, E. H., Lovelace, J., Pokross, M. and Borgstahl, G. E. O. "The High Mosaicity Illusion: Revealing the True Physical Characteristics of Macromolecular Crystals" Acta Cryst. D56, 986-995

Lovelace, J. Snell, E. H., Pokross, M., Arvai, A. S., Nielsen, C., Xuong N.-H., Bellamy, H. D. and Borgstahl, G. E. O. "BEAM-ish: a graphical user interface for the physical characterization of macromolecular crystals" J. Appl. Cryst. 33, 1187-1188 (2000).

Borgstahl, G. E. O., Pokross, M., Chehab, R., Sekher, A. and Snell, E. H. "Cryo-trapping the Six-Coordinate, Distorted-Octahedral Active Site of Manganese Superoxide Dismutase" J. Mol. Biol. 296, 951-960 (2000).

Garlitz, J. A., Summers, C. A., Flowers II, R. A. and Borgstahl, G. E. O. "Ethylammonium Nitrate: A Protein Crystallization Reagent" Acta Cryst. D55, 2037-2038 (1999).

Guan, Y., Hickey, M. J., Borgstahl, G. E. O., Hallewell, R. A., Lepock, J. R., O'Connor, D. Hsieh, Y. Nick, H. S. Silverman, D. N. and Tainer, J. A. "Crystal Structure of Y34F Mutant Human Mitochondrial Manganese Superoxide Dismutase and the Functional Role of Tyrosine 34", Biochemistry 37, 4722-4730 (1998).

Genick, U.K.*, Borgstahl, G.E.O.*, Ng, K., Ren, Z., Pradervand, C., Burke, P.M., Srajer, V., Teng, T.Y., Schildkamp, W., McRee, D.E., Moffat, K., and Getzoff, E.D. "Millisecond Time-Resolved Laue Crystallography: Structure of a Protein Photocycle Intermediate", Science, 275, 1471-1475 (1997) *These authors contributed equally to this work.

Ren, Z., Ng, K., Borgstahl, G. E. O., Getzoff, E. D., and Moffat, K. "Quantitative Analysis of Time-resolved Laue Diffraction Patterns", J. Appl. Cryst. 29, 246-260 (1996).

Borgstahl, G. E. O., Parge, H. E., Hickey, M. J., Johnson, M. J., Boissinot, M., Hallewell, R. A., Lepock, J. R., Cabelli, D. E. and Tainer, J. A. "Human Mitochondrial Manganese Superoxide Dismutase Polymorphic Variant Ile58Thr Reduces Activity by Destabilizing the Tetrameric Interface", Biochemistry, 35, 4287-4297 (1996).

Borgstahl, G. E. O., Williams, D. R., and Getzoff, E. D. "The 1.4 Å Structure of Photoactive Yellow Protein, a Cytosolic Photoreceptor: Unusual Fold, Active Site and Chromophore", Biochemistry, 34, 6278-6287 (1995).

Baca M., Borgstahl, G. E. O., Boissinot, M., Burke, P. M., Williams, D. R., Slater, K. A. and Getzoff, E. D. "Complete Chemical Structure of Photoactive Yellow Protein: Novel Thioester-linked 4-Hydroxycinnamyl Chromophore and Photocycle Chemistry", Biochemistry, 33, 14369-14377 (1994).

Borgstahl, G. E. O., Rogers, P. H., and Arnone, A. "The 1.9 Å Structure of Deoxyβ4 Hemoglobin: Analysis of the Partitioning of Quaternary-associated and Ligand-induced Changes in Tertiary Structure", J. Mol. Biol., 236, 831-843 (1994).

Borgstahl, G. E. O., Rogers, P. H., and Arnone, A. "The 1.8 Å Structure of Carbonmonoxy-β4 Hemoglobin: Analysis of a Homotetramer with the R-like Quaternary Structure of Liganed α2β2 Hemoglobin", J. Mol. Biol., 236, 817-830 (1994).

Borgstahl, G. E. O., Parge, H. E., Hickey, M. J., Beyer, W. F., Hallewell, R. A. and Tainer, J. A. "The Structure of Human Manganese Superoxide Dismutase Reveals a Novel Tetrameric Interface of Two 4-helix Bundles", Cell, 71, 107-118 (1992).