 |
Kathy Cleaves (again)* escaped from Creighton University.
She was acquired by UNMC in late 2002 and is an integral part of 10th floor
operations. |
|
Administrative Unit |
 |
The Jade tree has been a fixture in the lab
longer than anyone else except Gloria. |
|
Jade Tree |
 |
The Market Forge Autoclave would probably
better suited in a museum, however it allows small samples to be prepared in
the lab instead of fighting for time in the large autoclave a floor away. |
|
Autoclave |
 |
The lab has a New Brunswick Scientific Bioflo
4500 fermentor which is used to express large amounts of protein. The
system can make 20 liters at a time and is self sterilizing. |
|
Fermentor |
 |
The lab has a Sorvall RC5C plus centrifuge used
to pellet cells. It has the ability to run in flow through mode in
conjunction with the fermentor to rapidly pellet a large volume of sample. |
|
Centrifuge |
 |
The lab has a Thermo Spectronic Biomate 5
spectrometer to analyze sample concentration. |
|
Spectrometer |
 |
|
Electrophoresis System |
|
The lab has a Pharmacia Phastgel system to aid in the
analysis of sample purity. |
 |
The lab uses an Akta Explorer for HPLC work.
"It will automatically purify proteins." |
|
HPLC |
 |
The lab has a Protein Solutions DynaPro MS/X
dynamic light scatter system with a temperature controller. The system
helps screen protein preparations to determine if the samples are of a
quality that may favor crystallization. |
|
Dynamic Light Scattering System |
 |
A Model L-100 Grease Machine saves lots of time
greasing 24 well crystallization trays. |
|
Grease Machine |
 |
To analyze crystal trays there is a Meiji
microscope with 10x, 20x, or 30x optics and an attached Sony DKC-5000 "Cats Eye" 3
CCD digital camera. |
|
Microscope /w CCD Camera |
 |
The lab has an FR-E SuperBright x-ray source
which is a combination of existing rotating anode expertise and
state-of-the-art microfocus X-ray generation technology. The system
uses Osmic mirrors for focusing. |
|
X-Ray Generator |
 |
To the left port of the FR-E source is an RAXIS-IV
++ detector. This is used to check crystals for diffraction quality,
develop cryocooling protocols and solve structures. |
|
Area Detector |
 |
To the right port of the FR-E source is Enraf
Nonious precession camera that was donated by Schutt from the University of
Princeton and is used for challenging crystallographic
problems. Currently, it is being used to study the semicrystalline
state of profilin:actin. |
|
Precession Camera |
 |
The X-Stream 2000 system provides a low
temperature nitrogen stream (-180C) for cryocooling crystals with out the need for a liquid nitrogen
tank. |
|
Low Temperature Stream |
 |
The instruments to the left are universally
required for any real lab... |
|
Critical Research Instruments |
